Expression Pro le of Long Non-coding RNAs in Response to Ionizing Radiation

Long non-coding RNAs (lncRNAs) are a class of non-coding transcripts longer than 200 nt and potentially play important roles in eukaryotic gene regulation. However, the quantity, characteristics and expression pattern of lncRNAs responding to ionizing radiation remain largely unknown. We speculate that some lncRNAs participate in the induction of radiobiological effects and ionizing radiation changes their expression levels. In the present study, total RNAs of HeLa cells were isolated 4 h after exposure to 2 Gy of X-rays, and sequenced by lncRNA-seq technology. We identified 647 kinds of known lncRNAs and 381 kinds of novel non-coding sequences responding to ionizing radiation. Subsequently, we verified the expression of 24 lncRNAs after exposure to 2 Gy
X-rays or 80 keV/m carbon ions by using qRT-PCR.