Concentration of Signaling Factors Released by Cells Irradiated with Carbon Ions at Different Time Points Postirradiation
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Graphical Abstract
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Abstract
Signaling factors usually play an important role in bystander effect. In this work， human hepatoma QGY7703 cells in vitro were irradiated with highLET carbon ions. Concentrations of signaling factors such as TGF-β1 and NO were measured in the media of the irradiated QGY-7703 cells at different time points after irradiation. The conditioned media harvested at various times postirradiation were transferred to human hepatocyte L02 cells as bystander cells and then the influence of the conditioned media on survival fraction and cell viability of the bystander cells were determined. The results show that the irradiated cells regulate the concentration of the signaling factors released nearby themselves temporally and spatially， and the bystander cells response to the signaling factors differentially according to the concentration change. This work provides new basic data for exploring the bystander effect， especially caused by highLET radiation.
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