Culture of Threedimensional Tissue Model and Its Application in Bystandereffect Research
-
Graphical Abstract
-
Abstract
Compared with the cultured monolayer (2D) cells， threedimensional (3D) tissue could be more similar to the environment in vivo including the physical support， chemical factors， cellcell and cellmatrix interaction and so on. With the development of threedimensional cell culture techniques (TDCC)， 3D tissue is widely used in the areas of bystander effect research. This review focuses on introducing the TDCC method and its application in bystandereffect research. First， the development process of 3D tissue culture method was introduced. Secondly， the induction of radiation induced bystander effects both in 2D cell and 3D tissue and its mechanisms were reviewed. Finally， because heavy ion (carbon ion beam) has been developed as a useful tool to cure solid cancer ，and the 3D tissue model is an ideal material to study the damages on body after being irradiated and to understand the underlying mechanisms， future study about heavy ion radiation inducing bystander effect in 3D tissue was discussed.
-
-
DownLoad: