Research  on  Mutation  Breeding  of  Highproducing  Strains  from  Streptomyces  Avermitilis Irradiated  by  Ion  Beam  of <SUP>12</SUP>C<SUP>6+</SUP>

WANG  Shu-yan; CHE  Ji-hong; LI  Wen-jian; LIU  Jing; CAO  Yun-fei; BO Yong-heng; MA  Xiao-qi; LIANG  Jian-ping

doi:10.11804/NuclPhysRev.27.01.082

WANG Shu-yan, CHE Ji-hong, LI Wen-jian, LIU Jing, CAO Yun-fei, BO Yong-heng, MA Xiao-qi, LIANG Jian-ping. Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+[J]. Nuclear Physics Review, 2010, 27(1): 82-86. DOI: 10.11804/NuclPhysRev.27.01.082

Citation:

Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of ¹²C⁶⁺

Graphical Abstract

Graphical Abstract

Abstract

Abstract

Mutagenic effect on avermectin B1a producing strains of ZJAV-A1 by ion beam of ¹²C⁶⁺ has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%， when ZJAVA1 was irradiated by ion beam of 50 Gy ¹²C⁶⁺. After the mutagenesis processing by ion beam of ¹²C⁶⁺ and the screening of orthomutation strains by using plating mediums and slant cultures，the mutant ZJAV-Y1203 was obtained with theavermectin B1a yield of 4588—4460 μg/ml. Compared with the original strain， the titer was improved 11.1%—14.7%.

FullText(HTML)

References (0)

Cited By

Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+

Graphical Abstract

Abstract

Catalog

Export File

Citation

Format

Content

Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of ¹²C⁶⁺