Changes of Immune System in Mice Irradiated by Continual X rays
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Graphical Abstract
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Abstract
For estimating the effect of low doses Xray continual irradiation to immunity system of mouse， BALB/c mice were continually irradiated to 1.03 Gy by X rays at a dose rate of 0.2 Gy/min in 13 d. At 24 or 48 h after irradiation， the immunocyte cycle and apoptosis were determined by flow cytometry， and the thymus and spleen weights were measured too. The results showed that the cycle of thymocyte were arrested in G2/M at 24 h， the number of peripheral blood lymphocytes and thymocytes in G0/G1 phase at 48 h was up and the percentage of apoptosis had a significance increase in both of time points； the cycle of spleen lymphocytes was delayed in G0/G1 at 24 h， in S phase at 48 h， the apoptosis had a significance decrase at 24 and 48 h； spleen index declined significantly at 48 h. The results suggested that low doses continual Xray wholebody irradiation could activate different cell cycle checkpoints， and result in some changes of apoptosis and some damages to immunocytes. The continual Xray irradiation affects the organs differently， it might provide experiment basis for radioprotection.
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