Radiation induced Delayed Effect in Human Normal Liver HL-7702 Cells
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Graphical Abstract
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Abstract
Human normal liver cell line HL-7702 cells were irradiated with different doses of Xrays. Micronucleus and apoptosis rates in the irradiated cells were measured with cytokinesisblock micronucleus method and Annexin VFITC apoptosis detection kit， respectively. Experimental data showed that the micronucleus and apoptosis rates increased obviously with increasing irradiation dose. After seven population doublings， the micronucleus and apoptosis rates of the cells surviving exposure to the Xrays reduced to the same levels as nonirradiated control cells; the progenies of the cells were secondly exposed to Xrays at the same dose of 2.5 Gy. We found that the progenies of the cells surviving the first irradiations of the various doses showed markedly differential micronucleus frequencies and apoptotic rates. Although the same dose of 2.5 Gy was applied in the second irradiations， the micronucleus frequencies and apoptotic rates of the progenies of the cells initially exposed at higher doses were significantly higher than the others. These results indicate that Xrays lead to genomic instability in HL7702 cells， which is an important manifestation of radiationinduced delayed effect， and a second radiation stimulus makes the delayed effect in the progeny of the previously irradiated cells be expressed obviously.
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