Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation
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DU Bin-bin,
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LIU Qing,
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ZHANG Wei-sheng,
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WANG Xiao-yan,
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JIA Pen-fei,
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WANG Xiao-hu,
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LI Sha,
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YANG Xiong-fei,
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ZHANG Hong,
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Guo Tian-kang,
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Graphical Abstract
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Abstract
To investigate the role of NADPH oxidase in HeLa cell lesion induced by Xray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laserscanning microscopy was employed to detect the colocalization of two subunit of NADPH oxidase, p47phox and gp91phox in the cell. Western blotting was used to detect the expression of gp91phox before and after Xray irradiation. After Xray irradiation， intracellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47phox moved to membrane and colocalizated with gp91phox after
irradiation. Moreover, the results also show that gp91phox increased sharply after 12 Gy Xray irradiation. Therefore， NADPH oxidasemediated production of ROS plays an important role in HeLa cell lesion induced by Xray.
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