High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray
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Graphical Abstract
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Abstract
To determine whether lowdose preirradiation could enhance adenovirusmediated p53 transfer and expression in human prostate adenocarcinoma， the PC3 cells were preexposed to γ rays， and then infected with replicationdeficient adenovirus recombinant vectors， containing human wildtype p53 (AdCMVp53) or green fluorescent protein gene (AdCMVGFP) respectively (γray irradiation + AdCMVp53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMVGFP infection groups were 7%—39% more than those in AdCMVGFP infection groups. The p53 levels in the γ irradiation + AdCMVp53 infection groups were 18.5%—35.4% more than those in AdCMVp53 infection groups (p<0.05)，suggesting that lowdose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC3 cells. The survival fractions for the γ irradiation + AdCMVp53 infection groups were 25%—64%， 22%—65% less than those for AdCMVp53 infection， or γirradiation groups， respectively (p<0.05).
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