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DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. DOI: 10.11804/NuclPhysRev.24.01.055
Citation: DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. DOI: 10.11804/NuclPhysRev.24.01.055
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Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells

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  • The effect of 12C6+ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of 12C6+ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that 12C6+ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.
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