电离辐射引起的线粒体DNA损伤及突变研究进展
Current Study on Ionizing Radiationinduced Mitochondial DNA Damage and Mutations
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摘要: 指出了线粒体DNA损伤及突变研究与核DNA相关研究的区别,并总结了国内外关于电离辐射引起线粒体DNA损伤突变的研究进展。 针对放射生物学中线粒体学方面研究还有待进一步加强的现状， 中国科学院近代物理研究所的研究人员在方法学上建立起了real-time PCR和long PCR等手段对线粒体DNA损伤及突变进行定量检测， 并以此为基础， 对电离辐射引起的线粒体功能变化进行了较为深入的研究。总结了线粒体DNA损伤及突变研究对阐明电离辐射引起的生物学效应所具有的贡献， 提出未来在放射生物学中以远后效应， 能量代谢为主的线粒体学研究方向。 Current advance in ionizing radiationinduced mitochondrial DNA damage and mutations is reviewed， in addition with the essential differences between mtDNA and nDNA damage and mutations. To extent the knowledge about radiation induced mitochondrial alterations， the researchers in Institute of Modern Physics， Chinese Academy of Sciences developed some technics such as realtime PCR， longPCR for accurate quantification of radiation induced damage and mutations， and indepth investigation about the functional changes of mitochondria based on mtDNA damage and mutations were also carried out. In conclusion， the important role of mitochondrial study in radiation biology is underlined， and further study on mitochondrial study associated with late effect and metabolism changes in radiation biology is pointed out.Abstract: Current advance in ionizing radiationinduced mitochondrial DNA damage and mutations is reviewed， in addition with the essential differences between mtDNA and nDNA damage and mutations. To extent the knowledge about radiation induced mitochondrial alterations， the researchers in Institute of Modern Physics， Chinese Academy of Sciences developed some technics such as realtime PCR， longPCR for accurate quantification of radiation induced damage and mutations， and indepth investigation about the functional changes of mitochondria based on mtDNA damage and mutations were also carried out. In conclusion， the important role of mitochondrial study in radiation biology is underlined， and further study on mitochondrial study associated with late effect and metabolism changes in radiation biology is pointed out.
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